FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7345216
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG1917852.6110191994353875TruSeq Adapter, Index 20 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGC83930.11426484939312881TruSeq Adapter, Index 20 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA206250.044.3531951
AGTCACG215900.041.6275228
TCGGAAG221400.041.2847563
ATCGGAA221600.041.2782632
CTCGTAT219100.041.23516544
TATCTCG219300.041.15652541
TCGTATG219850.041.1558945
TCTCGTA219600.041.15152443
ACACGTC221950.041.1302613
CGTCTGA221950.041.0491816
ATCTCGT220250.041.01048742
GTCACGT219250.041.00118329
CGGAAGA223650.040.8593444
ACGTGGC221100.040.84126332
ACGTCTG223350.040.81201615
CGTGGCC221450.040.68528433
TCACGTG223050.040.61532230
CACGTCT224550.040.61395314
GCACACG226500.040.34375411
CACACGT227350.040.20281612
Produced by FastQC (version 0.11.5)