FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7076289
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3600.023.1237245
TCTCGTA5100.017.64745340
CTCGTAT5200.016.44209341
CGGCTAC5050.015.59297532
CGTATGC5500.015.54525243
ACGGCTA5100.014.99895831
TCGTATG5750.014.8693742
CGTTGGA21350.013.8111991
GTCACGG6150.013.53564428
ATCTCGT6500.013.154139539
ACACGTC6850.013.13897912
ATCGGAA6950.012.9548721
ACGGGAT12800.012.8313199
AAACGGG22750.012.6586397
AGTCACG6600.012.61275927
TCGGAAG7200.012.5050522
GAAACGG23150.012.43965156
TCACGGC6750.012.33247629
CATCTCG6950.012.30234538
AACGGGA19050.012.2827848
Produced by FastQC (version 0.11.5)