FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8860919
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA1005681.1349612833612406TruSeq Adapter, Index 2 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC98300.1109365744117512TruSeq Adapter, Index 4 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA105850.029.3832021
ATCGGAA116100.026.7410492
ACACGTC116050.026.5968813
CGTCTGA116100.026.54727416
TCGGAAG116950.026.5210423
CGGAAGA117200.026.4254824
CACGTCT117700.026.23765814
ACGTCTG117550.026.220115
GCACACG118000.026.19533711
CACACGT119000.026.02562112
AGCACAC126500.024.5774510
AGAGCAC126450.024.4920048
GAGCACA127200.024.3596619
CAGTCAC128200.023.98233227
TCTGAAC129350.023.97863218
GAAGAGC130000.023.9385536
GTCACTG128700.023.80731829
AGTCACT130100.023.66640728
CCAGTCA129850.023.65448826
GTCTGAA131500.023.58658617
Produced by FastQC (version 0.11.5)