FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7178416
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA277950.38720241345723067TruSeq Adapter, Index 3 (100% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT77220.10757247838520363TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA41350.023.9818731
AGATCGG9850.023.5488221
ATCGGAA43350.022.8745352
ACACGTC43200.022.57160813
TCGGAAG43900.022.518773
CGTCTGA43350.022.49381616
ACGTCTG43750.022.42510815
CGGAAGA45150.021.994854
GCACACG44400.021.96156511
CACGTCT44500.021.94617714
CACACGT46100.021.18419312
TCACTTA51400.019.86165430
AGAGCAC51350.019.368368
AGCACAC53700.018.9391610
GAGCACA53600.018.6950459
TCTGAAC53650.018.48283818
CAGTCAC53950.018.41988627
CCAGTCA53850.018.36841226
GTCACTT54250.018.319329
GAAGAGC55050.018.2026256
Produced by FastQC (version 0.11.5)