FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6621212
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA6950.022.0128232
CGGCTAC7100.021.2308833
CTCGTAT7450.020.53482842
TCTCGTA7550.020.26330241
CGTATGC7650.019.40979444
GATCGGA8750.018.0490131
TCGTATG8300.017.8897543
ATCTCGT8500.017.7337640
GTCACGG9150.016.71986829
CATCTCG8950.016.59074639
CACGGCT9200.016.14015431
TCACGGC9950.015.60190230
AGTCACG9950.015.37555528
ATCGGAA10300.015.2945612
CGTCTGA10350.015.21607616
TCGGAAG10200.015.0031233
ACCGCGC4850.014.84430231
ACACGTC10950.014.38188513
CGCGCCC3650.014.17707133
CACCGCG5650.013.93705830
Produced by FastQC (version 0.11.5)