FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8303104
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA23000.012.6430031
ACCGCGC6150.012.43563731
CCGCGCC6650.012.17713432
AACGGGA23100.012.0746348
ACGGGAT16800.012.0502889
CGCGCCT2654.8621587E-811.88355533
GAAACGG25800.011.7711846
CGCGCCC4800.011.71555933
AAACGGG26750.011.6884057
CACCGCG6700.011.41480230
CAGCGCG1400.005271517711.24693712
CGGGATT15700.011.03202610
GGAAACG27050.010.9991315
GATTTCG10800.010.226435
GCCACCG18750.09.59738628
CCCGAAT6900.09.4740861
CCCGCAA3104.521513E-69.4530091
CCCGATT3603.1302443E-79.3924131
GCGTGAG21050.09.29692322
CCACCGC15250.08.85004829
Produced by FastQC (version 0.11.5)