FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6586368
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA15900.013.2293341
ACGGGAT10550.012.3674999
CGGGATT10050.011.49052110
GAAACGG18450.011.4675866
AAACGGG17850.011.4274357
CACCGCG4100.011.34016330
AACGGGA14400.011.1438788
CGAAACG1355.6291887E-511.116425
GGAAACG18100.010.7786285
GCCACCG9800.010.71256928
GATTTCG5850.010.2613125
ACAGGCG14850.010.09923918
GCGTGAG11700.09.99824622
GTCACGA1851.28446645E-59.72861529
GATCGGA1757.29975E-59.4441241
CCCGTAA1757.29975E-59.4441241
AACGGGT4500.09.33169658
GAGCCAC31750.09.2583626
CGTGAGC12050.09.21014323
CCCTAGA9600.09.0774491
Produced by FastQC (version 0.11.5)