FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8574719
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA24950.015.2733981
GAAACGG27450.013.3599996
ACGGGAT15450.012.9611329
AACGGGA22750.012.9560138
AAACGGG28200.012.6853997
GGAAACG29650.012.5204655
CGGGATT12900.011.68601710
ACGGGTT6400.010.8984119
CCCGCGT1450.006721975310.88552951
CGGGATA5050.010.69304410
CGGGAAT10250.010.53655910
AACGGGT7050.010.5318898
CCCGAAT8600.09.701141
ACCGCGC6050.09.29690131
CACCGCG7050.09.25470330
GTTGGAA51700.08.6172
CGCGCCC4406.5379754E-78.18127333
CGCCCGG8450.07.987633735
CGGGTTT7507.2759576E-127.799980610
ACGGGAA11550.07.79218869
Produced by FastQC (version 0.11.5)