FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7491889
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA9000.025.5932481
ACACGTC9600.023.7162213
ATCGGAA10000.023.6672422
TCGGAAG9950.023.3331343
CGTCTGA10400.022.18009616
ACGTCTG10850.021.39823715
CGGAAGA11350.020.5870064
CACGTCT11850.019.33967214
GCACACG12150.019.10820411
CACACGT13400.017.32583812
AGAGCAC19450.012.4755548
CGTTGGA16000.012.2562271
AGCACAC20800.012.025910
CACCGCG6600.011.58393430
GAAGAGC20950.011.367836
GAGCACA21600.011.1644319
CAGTCAC20500.011.11332727
CGTGAGC16050.010.82844923
GTCACAT21900.010.74666629
CCAGTCA21900.010.67564926
Produced by FastQC (version 0.11.5)