FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7862589
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA7950.029.4569971
CTCGTAT8050.028.2274744
TCTCGTA7900.028.19386343
ATCGGAA8700.027.1724472
AACGATC9050.025.35701438
TCGGAAG9350.025.0424983
ATCTCGT9250.024.32230842
ACACGTC9900.023.4071513
CGTCTGA10500.022.49813516
GTCACGT10300.022.27970529
AGTCACG10350.021.95469928
CGGAAGA10800.021.6792094
TCGTATG10500.021.21360445
AAACGAT10800.021.03992337
ACGTCTG11400.020.52461215
ACGTGAA12050.018.67065232
CGTGAAA12450.018.61291533
GAAACGA12950.018.4154636
CACGTGA12450.018.251531
CACGTCT12950.017.72053714
Produced by FastQC (version 0.11.5)