FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6412046
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3601.8189894E-1111.87347544
CACCGCG6250.011.1584830
GCGTGAG15950.011.14320422
CTCGTAT3907.8216544E-1110.96013142
GCCACCG15350.010.70003728
TCTCGTA3951.1241355E-910.25184741
CGTGAGC16500.010.22771523
CGTTGGA18300.09.8516261
CGCGCCT3206.538485E-79.84240933
ACGGGAT12350.09.4724679
ACAGGCG23300.09.46193518
TCGTATG4854.1654857E-109.2771643
ACCGCGC6400.09.139379531
AGGCGTG23400.09.13372820
CCGCGCC6700.09.06592832
AACGGGA16900.08.9186258
CGCCAAT4551.2836608E-88.89988733
AAACGGG19500.08.8836827
ACGCCCG3053.9205108E-58.85125334
GGCGTGA21950.08.81463221
Produced by FastQC (version 0.11.5)