FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9805717
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC7600.011.5440831
CGTTGGA22400.011.375481
GCCACCG22400.011.34844928
ACGCCCG4950.010.90722734
TATGCCG4405.456968E-1210.748753
AACGGGA18800.010.6495048
GCGTGAG24650.010.58669622
CGTGAGC25550.010.38987823
GCGCGAT3755.2277755E-910.19799720
CCGCGCC8200.010.15070432
CACCGCG8750.010.02685730
TGGCGCG3603.018613E-89.99803518
GGCGCGA3858.050847E-99.93311419
ACGGGAT14400.09.6855969
CGCGCCC6150.09.5105733
AAACGGG24400.09.4976357
GAGCCAC70250.09.382977526
GAAACGG25700.09.3748996
AGGCGTG31350.09.25655520
CGCGATC4152.7384885E-89.2154821
Produced by FastQC (version 0.11.5)