FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8353258
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA17200.011.5985818
ACGGGAT13150.011.4065239
CGTTGGA19850.011.1396761
ATAGGCG3100.011.12752118
AAACGGG21100.011.0898027
CGGGATT13250.010.64038210
GCTATCG1000.00878430710.5000815
GCGTGAG14500.010.23998822
ACAGGCG21950.010.04419318
CGTGAGC15200.09.7684123
GAAACGG22950.09.7389296
GAGCCAC40500.09.59134726
CCCAAAG66300.09.4383371
GGAAACG23300.09.3992155
CCCCGAT1809.803154E-59.1925481
TAGGCGT3101.9117579E-99.192319
CCCGAAA2209.871706E-68.8886621
AGGCGTG19200.08.67069620
CGGGAAT6750.08.66574910
CCCCGAA2701.6862687E-68.3568611
Produced by FastQC (version 0.11.5)