FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6051597
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT3100.020.31087744
TCTCGTA3350.018.79743
TATCTCG3850.015.77169641
TCGTATG4450.015.70626745
GATCGGA3900.015.6560451
ATCGGAA4450.015.2166322
ATCTCGT4200.014.992846542
TCGGAAG4600.014.7204373
ACGGGAT12000.013.6791649
ACACGTC5100.013.66814213
CGTTGGA19300.013.4748331
AACGGGA15200.013.4621918
AAACGGG19250.013.1997497
CGGGATT11150.012.50359210
CCGGCTA5750.012.12455138
GGAAACG20500.012.1114495
GTCCGGC1506.485961E-411.99338436
ACCGCGC4200.011.78155431
CGGTTCA1350.003916722311.7036564
GAAACGG21150.011.7004146
Produced by FastQC (version 0.11.5)