FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6611747
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA19200.012.2220661
CCCCGAT1551.4947855E-611.645841
ACGGGAT11800.011.5653389
AAACGGG20300.011.3030167
AACGGGA15900.011.224038
GGAAACG21050.010.7624475
CGGGATT11150.010.62554510
GAAACGG21950.010.3894376
CTAGACG1451.1708439E-410.34262911
GCGTGAG9450.09.99825322
CCGAGTA6950.09.4989112
ACGGGTT3851.8189894E-129.3486629
GCTAGAC9200.09.29262829
ACAGGCG14050.09.07294218
AACGGGT5050.08.90899858
GAGCCAC29950.08.71372726
CCCTAAT10200.08.7010781
CCCAAGT20950.08.6162541
CCCCGGA2105.5283133E-58.5957381
GCCACCG8900.08.59471928
Produced by FastQC (version 0.11.5)