FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8298757
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA4350.017.58473642
CTCGTAT4550.016.31731443
CGTATGC5650.015.13111245
ATCGGAA5850.013.4866581
CGTTGGA25700.012.9813781
CGTCTGA7700.012.27140415
ATCTCGT6650.012.18009341
AACGATC6750.011.99971937
TCGGAAG6950.011.9861542
AAACGGG26950.011.9378487
ACGGGAT16950.011.81409559
TCGTATG6600.011.58972944
AACGGGA22750.011.5713678
ACACGTC7050.011.48902312
AGTCACG7300.011.40322327
GAAACGG28700.011.2099316
GGAAACG29250.011.0773315
GTCACGT7350.011.01955228
CGGGATT17450.010.70194810
CGCGCCC3806.4810592E-910.0652533
Produced by FastQC (version 0.11.5)