FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6925313
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA20200.014.9451161
CGCCCGG6300.013.92731935
AAACGGG21350.013.5936257
ACGCCCG3850.013.44034834
ACGGGAT13000.013.3257249
GAAACGG22500.012.898846
GGAAACG22900.012.8768075
ACCGCGC3850.012.85598531
AACGGGA18050.012.838188
CGGGATT11850.012.34066110
TGCGCCC4750.011.36739733
CCGCGCC4400.011.24898832
ACCACGC12700.011.16041231
CGTATAA5900.011.0736771
ATAGGCG2452.9057228E-611.01941718
GCGCGAT2056.4148146E-510.97462220
TGGCGCG2301.7389502E-510.759918
CACGCCC10150.010.63943733
CCGGCTA6400.010.54592538
CACCGCG4751.8189894E-1210.420114530
Produced by FastQC (version 0.11.5)