FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6548545
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAG10900.012.79581422
CGTGAGC11300.012.47577423
GATCGGA1856.85759E-912.1838351
ACAGGCG14000.011.78354818
CACCGCG3250.011.53713330
CGTTAAT2002.174238E-811.2700471
ATCGGAA2301.3060344E-911.0922872
CGTTGGA20200.010.7865151
GAGCCAC30450.010.7863626
GGCGTGA13250.010.7527421
ACACGTC2251.10649125E-810.66470613
ATAGGCG1851.125467E-610.53860418
ACGGGAT12400.010.5222599
AACGGGA17500.010.3695238
CGGGATT11250.010.13147110
AGGCGTG14400.010.10230920
AAACGGG22100.09.9763047
CCCAAAG49200.09.9261941
CAGGCGT14100.09.89179919
TCGGAAG2454.109279E-89.8006293
Produced by FastQC (version 0.11.5)