FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6891263
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA20600.014.9173851
ACGGGAT13250.013.5829479
AAACGGG22950.013.2333867
AACGGGA18150.013.1386028
GAAACGG23700.012.7196856
CGGGATT12750.012.35187710
GGAAACG23400.012.3059185
AACGGGT5100.010.586718
GATTTCG8750.09.2558085
ACGGGTT4254.029971E-88.9987039
CGTTAAT2300.0021446038.8416981
ACAGGCG24500.08.63184618
CCCGAGA4752.4811015E-88.5624871
CGAAGAT6603.6379788E-128.5588841
CCGAGTA13500.08.4990222
CGGGATA2658.4756606E-48.48983510
GCGCGAT2658.476271E-48.48977420
GGCGCGA2658.476271E-48.48977419
CGCGATC2700.00102037248.33249521
GTCGTTT3003.171519E-48.249052
Produced by FastQC (version 0.11.5)