FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5510650
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4750.025.57359144
TCTCGTA5100.023.3774643
GATCGGA5000.022.9862841
TCGTATG5750.019.96466345
ATCGGAA6000.019.530832
ATCTCGT6050.019.33479542
TCGGAAG6450.018.515753
ACACGTC7100.018.05959913
CGGAAGA6900.017.30824
CGTCTGA7250.016.13455416
AATCTCG7950.014.99686141
ACGTCTG8450.014.64190615
CGTTGGA15250.014.4818751
GCACACG8750.014.39698711
CACGTCT9100.014.09045814
AAACGGG15750.013.8542417
ACGGGAT9200.013.6927859
AACGGGA13600.013.2325258
GAAACGG17350.012.8380276
CACACGT10250.012.50957712
Produced by FastQC (version 0.11.5)