FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7805237
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4850.025.5305331
TCTCGTA5550.022.7011241
CTCGTAT5650.021.90112942
CGTATGC5900.020.59178744
TCGTATG6400.020.38920443
ATCGGAA6450.020.243342
TCGGAAG7450.017.5260013
ATCTCGT7050.017.23284140
TCACGAT7600.016.87382535
CGTCTGA8200.015.91352716
ACACGTC8050.015.65108513
CATCACG9600.014.06152233
CGTTGGA20300.013.9737641
ACGTCTG9550.013.89955515
CACGATC9800.013.77455136
AACGGGA17450.013.6666718
ACGATCT9850.013.4762237
CACGTCT9850.013.24780914
CGGAAGA9550.013.2003244
AAACGGG23650.012.36700257
Produced by FastQC (version 0.11.5)