FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6287255
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA22750.014.7999951
AAACGGG23000.014.7630647
ACGGGAT13750.014.5547369
AACGGGA20050.014.3553118
CGTCCGA950.005398559414.2029716
CGGGATT13500.013.65831510
GAAACGG24450.013.5204166
ACCGCGC4400.013.28849231
GGAAACG25150.012.754035
GCCACCG12800.012.64851528
CGCGCCC3405.456968E-1212.56694433
CACCGCG4850.012.51801930
CCACCGC10550.011.2963229
CCGCGCC5200.011.24410932
CGGGAAT8950.011.05469210
CCCGAAC2056.1334795E-511.0230781
ACAGGCG20500.010.63975118
CGTGAGC15250.010.32221423
GCTAGAC14900.010.262129
GCGTGAG14750.010.21474322
Produced by FastQC (version 0.11.5)