FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4655558
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC3850.015.1943231
AACGGGA11250.014.399238
ACGGGAT8450.014.377939
CGTTGGA13000.014.3690921
AAACGGG13550.013.2838517
GAAACGG13700.012.6458526
CGGGATT7550.012.51588610
GGAAACG14250.012.4735545
CACCGCG4550.011.86749730
CGGGAAT4300.011.51101210
CCGCGCC5000.011.24976132
CGCCCGG5850.011.153728535
CGAAACG1500.00878731410.49943810
ACGCCCG3309.0527465E-810.227056534
CGGGATA1800.0029580589.99946610
CCCGAAG2658.2188584E-59.3420331
CGGCTAA5454.3655746E-119.08344939
CCCGTAA2000.0070348379.0023221
AAGCGGC4304.8494257E-88.8963063
CGGCTGA4355.858783E-88.7930126
Produced by FastQC (version 0.11.5)