FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13627403
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGA1257.4760464E-1021.6142481
CGTTGGA38400.014.5994661
AACGGGA38300.014.039738
AAACGGG45050.014.0340287
ACGGGAT27000.013.9159349
GAAACGG48550.013.2553326
CGGGATT25650.012.80662410
GGAAACG49750.012.3495735
CGGGAAT16700.010.91283910
CCCGAAA6650.010.4956411
CCCGATA2902.1446407E-59.3164861
AACGGGT10150.09.30985458
ACCGCGC8850.09.15216131
GTCGGAA4458.718416E-99.1036172
ACGGGAA18900.08.9281029
CACCGCG9600.08.90587830
CCCGGAA7350.08.8834071
GCGTGAG33300.08.51297122
GATTTCG17350.08.4313345
CCGCGCC10200.08.38175732
Produced by FastQC (version 0.11.5)