FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8805638
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1082231.2290194077930525TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA115700.043.3505941
ATCGGAA123100.040.7527352
CTCGTAT121600.040.57114842
TCGGAAG124050.040.493973
CGTATGC122050.040.47685644
TCTCGTA122150.040.3700541
ACACGTC123850.040.3061213
TCGTATG122800.040.19343
ATCTCGT123150.040.06050540
CGTCTGA124550.039.84478816
CGGAAGA127250.039.7232554
ACGTCTG126450.039.31725315
AATCTCG126150.039.1432639
CACGTCT127350.039.07472214
GTATGCC127050.038.90555645
GCACACG129700.038.64425711
CACACGT130400.038.38505612
GTCACAC138400.035.80866629
AGAGCAC144700.035.104698
GAATCTC141650.034.8917938
Produced by FastQC (version 0.11.5)