FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5318279
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCAA1507.240487E-813.0216651
ACGGGAT9700.012.059929
AACGGGA12950.012.0443998
AAACGGG15300.011.6661127
CGTTGGA14800.011.4717881
CCGCAAC1059.6896273E-411.4329722
CGGGATT8700.011.37748410
GAAACGG16400.011.1617836
GGAAACG16250.010.9888455
CCCCGAA1501.6181055E-410.0166651
GCGTGAG8200.09.69356622
GCCACCG8250.09.63499828
AACTACG1250.00413127859.6036975
CGCTAGA1250.0041492819.5986428
CCCTAAA12750.09.5452931
ACAGGCG10850.09.5376418
CGTAGAT1603.0975335E-49.3906231
CGTGAGC9500.09.31446923
CCCAAAG40850.09.2687861
CGCCACC4050.09.25794827
Produced by FastQC (version 0.11.5)