FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6749980
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGA4650.029.51017636
CGTCCCG4750.028.88890835
CTCGTAT5200.026.82151644
CCGATCT5300.026.31544739
TCGTATG5450.025.6012245
TCTCGTA5450.025.5911743
TCCCGAT5500.025.35852237
CCCGATC5500.024.94951238
GATCGGA6500.022.536531
ACCCGTC6750.020.662532
CACCCGT7000.020.24591631
ATCTCGT7150.020.135842
ATCGGAA7400.020.099852
CGTCTGA7350.019.28182416
TCACCCG7550.019.06900230
TCGGAAG7800.019.06643
CCGTCCC7750.018.576934
CCCGTCC7850.018.3402533
ACACGTC7750.018.28663613
ACGTCTG8500.016.40845515
Produced by FastQC (version 0.11.5)