FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9440271
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA5700.013.81550641
CTCGTAT5250.013.7141542
TCGTATG5100.013.67633543
CGTATGC5700.013.02604944
CCCGTAA4200.012.8642391
ACGGGAT17600.012.6556569
CGTTGGA26950.012.3630341
AACGGGA24100.011.949718
CGGGATT17000.011.77892110
AAACGGG29750.011.2684977
CGGGATA3602.237357E-1011.24953210
ATCTCGT6650.011.16524540
GAAACGG30000.010.5755126
GATCGGA7150.010.0754881
GGAAACG31100.010.05798155
GATTTCG11750.09.1930545
ACACGTC8450.09.05306913
TATGCCG3104.727953E-58.7112983
CGTCTGA8950.08.54726616
CCCGAAA4001.458E-68.4421561
Produced by FastQC (version 0.11.5)