FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10494688
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8550.029.47210344
TCTCGTA9000.027.49852643
GATCGGA9500.024.6468141
TCGTATG10750.023.85941145
ATCTCGT11250.022.59835842
ATCGGAA10850.021.9889772
AGTCACG11600.021.52901628
AACGATC11500.021.32626238
GTCACGT12450.020.42078629
CGTCTGA12400.020.32323316
TCGGAAG11900.020.0438063
ACACGTC12850.019.61152513
AAACGAT14050.017.77594837
CGGAAGA14750.016.4760484
CACGTCT16650.015.67617914
ACGTCTG16550.015.63494315
ACGTGAA16500.015.54476832
ACGATCT16850.014.82208239
TGAAACG17800.014.78987135
GCACACG18050.014.58419211
Produced by FastQC (version 0.11.5)