FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703176
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA9250.016.3308011
CGAAACG950.005345414.2239555
AACGGGA9300.013.7875138
CGGGATA1352.6590194E-413.33061710
GAAACGG12200.013.2892626
AAACGGG11350.013.2821027
ACGGGAT6850.013.1360099
CGGGATT6250.012.23750610
GATCGGA2054.7243757E-612.097991
CGGGTAT1701.3819695E-411.90933810
GGAAACG12700.011.8812965
CTCGTAT1902.955983E-511.84033342
AACGGGT2106.3389034E-611.7833148
ATCGGAA2452.8687045E-611.0308232
CGTCTGA2452.908495E-611.01816216
ACGGGTA1450.00683974410.8598569
ATCGTTC1903.8776043E-410.65601239
CGTATAT2352.1531843E-510.5535651
CTCGACA1500.00879565910.49800232
TCTCGTA2251.6481541E-49.99823341
Produced by FastQC (version 0.11.5)