FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10129993
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA35050.014.977751
ACGGGAT22050.014.589099
AACGGGA30450.013.8151328
AAACGGG37500.013.6175047
GAAACGG38700.013.2558066
CGGGATA5550.012.97033910
GGAAACG40100.012.6965235
CGGGATT21000.012.31892810
CGCCCGG13400.011.41564835
CGCGCCC7200.010.3106133
CCCGATT4152.575689E-99.7724421
GCGTGAG29000.09.54116622
ACCGCGC8750.09.51254231
CGGGAAT12850.09.45333310
ACGCCCG6500.09.34430234
GATTTCG14050.09.2997745
GTTGGAA65850.09.2037882
ACCACGC22600.09.15761431
CGTGAGC30250.09.14690323
CGCCAAT2801.4794756E-48.8514281
Produced by FastQC (version 0.11.5)