FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8415402
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA28400.013.8262691
AACGGGA23400.012.494928
ACGGGAT16900.012.2435439
AAACGGG29350.012.1841437
GAAACGG31000.012.044856
GGAAACG31800.011.56261355
ACGCCCG5350.011.35126834
CGGGATT16200.011.10659610
CGTTAAT3907.2759576E-1110.9941861
CACCGCG5650.010.74854530
CCGCGCC5450.010.73028532
ACCGCGC5050.010.68942331
CGCGCCC3805.7661964E-1010.6542633
CGTGAGC19900.010.51105223
GCGTGAG20150.010.04560322
ACAGGCG27700.09.82452618
CGCCCGG8950.09.80065935
CCCAAAG96950.09.6832161
CCGGCTA8200.09.59998438
CCCGAAA3858.108145E-89.3784991
Produced by FastQC (version 0.11.5)