FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5496168
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAG950.00526403514.2570581
ACGGGAT9150.014.0143179
CGTTGGA15450.013.58803751
AACGGGA13050.013.2738948
GAAACGG16150.012.6762556
AAACGGG15800.012.3874047
CGGGATT9850.012.33342210
GGAAACG17050.011.7432355
CCTCGAA2606.458232E-59.5504021
CCTCGGT3104.439895E-69.466381
CGGGAAT5801.8189894E-129.309134510
CGTTGAA2952.5436393E-59.1825121
CGTGAGC12400.09.07181523
CCCCGAT2250.00177149729.029471
CCCCGAA2250.00177149729.029471
GCGTGAG11900.08.88580422
TTCGGCT2050.0086409568.77934613
CCCTAGA11750.08.453121
TCGCATA2400.00327938.43678723
ACCGTGC4904.528738E-88.26423231
Produced by FastQC (version 0.11.5)