FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8263441
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA20100.013.0973558
CGTTGGA23450.013.054041
ACGGGAT15550.012.2990579
AAACGGG25150.012.1665057
CGGGATT15800.011.10801810
GAAACGG26550.010.84695156
CGTGAGC20000.010.68661323
GCGTGAG19650.010.64797122
GCGACAA1700.00184079110.58735722
GGAAACG26850.010.39158255
CGCGCCC5050.010.24717133
ACAGGCG29550.010.20219918
ACCGCGC7150.09.75455431
CCGCGCC6950.09.71165932
CACCGCG7200.09.68681330
CGCCCGG8750.09.257606535
GCGCCCG7350.09.18335634
CGGGAAT9600.09.14097310
GCCACCG17650.08.92282728
CCGGCTA8850.08.89875138
Produced by FastQC (version 0.11.5)