FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7524838
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3000.013.4883241
ATCGGAA3450.012.5914212
ACACGTC3900.011.52234913
CGTTGGA17800.011.1916951
TCGGAAG3750.011.1842843
CGATACA1000.006681159610.8944171
TCACCCG4000.010.86739530
CGGGATT10450.010.75044510
AAACGGG19400.010.7323437
ACGGGAT11950.010.6544859
AACGGGA15500.010.4369488
GAAACGG19600.010.3935596
CCACGAT3056.730261E-1110.2055421
GTCGGGA2803.092282E-1010.1642897
GCCACCG14250.010.09665228
CGTCTGA4250.09.86861816
GGAAACG20350.09.7896845
CCCAAAG62850.09.6575111
GCGTGAG14600.09.64571422
CCCTAAA17300.09.625951
Produced by FastQC (version 0.11.5)