FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8203450
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA27100.013.0464571
AAACGGG28050.012.0213387
ACGGGAT17350.011.9201789
AACGGGA22800.011.8315278
GAAACGG28900.011.1244266
CCCCGAA3906.730261E-1111.0414541
CGCCCGG7150.011.00515435
GGAAACG30100.010.9872475
CGGGATT16350.010.31188210
CGGGATA4501.0186341E-109.99106710
CACTGCG8850.09.65238730
ACGGGTT6100.09.2130759
CTGCGCC7450.09.05230932
CGTGAGC20700.09.0136823
ACTGCGC7850.08.87741331
GCGTGAG20300.08.85907522
AACGGGT7200.08.7421848
GCGCCCG6259.094947E-128.63228234
TGCGCCC5007.1413524E-98.54236233
ACCACGC14500.08.52685931
Produced by FastQC (version 0.11.5)