FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9277479
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA706710.7617478843121067TruSeq Adapter, Index 7 (100% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG605280.6524186150138416TruSeq Adapter, Index 7 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA65900.029.2098431
CACCAGA90100.023.9194430
ATCGGAA139250.015.6515421
ACACGTC138700.015.61426112
TCGGAAG140350.015.5588452
CGTCTGA139400.015.52559715
ACGTCTG139400.015.52559714
CGGAAGA140700.015.5094813
CACGTCT140750.015.39733213
GCACACG142400.015.26121410
CACACGT143950.015.107306511
AGTCACC147850.014.50576927
GAGCACA151500.014.4829728
AGAGCAC151200.014.4720327
GTCACCA148900.014.46375628
GAAGAGC153150.014.3267055
TCTGAAC152400.014.26997317
AGCACAC153300.014.2250229
CAGTCAC153300.014.06833726
TCACCAG154900.013.97128229
Produced by FastQC (version 0.11.5)