FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6455357
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT850.002571025615.87851731
ACGCTAG1250.002258477512.59627327
CACCGCG4900.012.39496430
AAACGGG20850.011.7565617
CGTTGGA19400.011.6376041
ACGGGAT12800.011.4199259
GGAAACG21500.011.3356035
AACGGGA17750.011.27590758
ACCGCGC4201.8189894E-1211.24728231
CGGGATT12100.011.15130610
GAAACGG21850.011.0153896
CCCGATT2301.6727696E-510.7976731
CGCATAG2402.6766409E-510.3477691
CGCGCCC3552.438901E-810.13627433
CCGTGCC6700.010.070086532
GCCACCG14200.09.97944228
GATTTCG9050.09.9740315
CGCCCGG5700.09.8640535
AACGGGT4203.270543E-99.6379148
CCGCGCC4702.3101165E-109.57015332
Produced by FastQC (version 0.11.5)