FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7197609
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC8750.031.6259944
GATCGGA9050.030.6048281
TCTCGTA9000.030.49750941
TCGTATG9300.029.5137243
CTCGTAT9650.028.44327442
ATCGGAA10200.027.3702932
TCGGAAG10450.026.9303873
CGTCTGA11350.024.77771216
ACACGTC11400.024.66903713
ATCTCGT11100.024.52502440
ACGTCTG12950.021.89010615
CGGAAGA12650.021.8895234
CACGTCT13250.021.22468214
GTATGCC13400.021.15645845
AATCTCG13650.020.2730739
GCACACG14700.019.89633411
CACACGT15550.018.23002212
CGTTGGA20150.016.4276581
AAACGGG22550.013.7682777
AACGGGA18750.013.5588928
Produced by FastQC (version 0.11.5)