FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6485544
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT13300.015.7307139
CGGGATT12550.015.41600610
CGTTGGA20300.015.3227961
AACGGGA17200.014.9105448
AAACGGG20650.014.2714597
GAAACGG21900.013.5643076
GGAAACG22650.013.3202425
CCCGGAT2451.5817932E-812.8800321
CGCCCGG5750.012.51985435
CCCGAAC2054.7404574E-612.0946641
ATCGTTC3001.7462298E-911.99819339
ACGCCCG3052.8543582E-811.06390834
CGCTAGA1450.00683797910.86043428
ATCGCGC2707.9067104E-710.83170137
GGATCGT3154.587673E-810.71267237
CGCTAAT1700.001812389210.6070841
CGCGCCC3909.0221874E-1010.38305233
ACCGTGC5900.010.29505931
CGGCCTA1750.002343723310.28416539
CGTCTAT2201.2881175E-410.245481
Produced by FastQC (version 0.11.5)