FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7223030
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCG3450.013.03685234
CGATAAG1454.6831908E-412.4666971
CGCCCGG7200.011.55662535
CCCGAGA4152.237357E-1010.3451051
CGTTGGA22750.010.2302261
GGCGTAA1850.00372723659.72418121
ACAGGCG24050.09.63014518
AACGGGA19200.09.6033638
AGGCGTA2352.5649153E-49.56887520
CCCTAAT15150.09.5454581
AAACGGG22000.09.5053477
ACGGGAT14500.09.4595969
CAACGTC2150.00116171439.4570333
GAAACGG23750.09.2784666
ACCGCGC5352.910383E-119.247731
CGTAAGC2453.8943012E-49.17862723
CCGCGCC6700.09.06268332
GCGTGAG17650.09.04570522
CGCGCCC5007.4578566E-108.99555233
ACCACGC13400.08.894793531
Produced by FastQC (version 0.11.5)