FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6200990
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGC70880.11430432882491345TruSeq Adapter, Index 18 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC10950.035.5823945
CGTCCGC11800.032.59726332
ACGTCCG11850.032.26989731
CTCGTAT12350.031.69197543
TCCGCAC12400.030.29436134
TCTCGTA13100.030.0492642
GTCCGCA12650.029.87347833
TCGTATG13050.029.64728744
CGCACAT13750.027.81078536
CCGCACA14050.027.05686235
ATCTCGT14400.027.02406341
ACACGTC15500.026.84769812
GTCACGT14500.026.8376928
AGTCACG14050.026.25636327
ATCGGAA15300.026.107751
CATCTCG15050.025.85690940
TCGGAAG15950.025.1478542
ACGTCTG16950.024.41828314
TCACGTC15800.024.34479129
CACGTCC16100.024.1705930
Produced by FastQC (version 0.11.5)