FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8221077
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG667300.8116941369117453TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG75300.042.23158641
GATCGGA76950.041.986471
TCTCGTA75650.041.9767443
CTCGTAT75650.041.8547744
TCGTATG75900.041.74456445
ATCTCGT76950.041.44293642
ATCGGAA78100.041.4023062
GTCACGT77800.041.0160729
TCGGAAG79100.040.907443
AGTCACG78000.040.8818228
ACACGTC79750.040.57614513
CGTGGCC79250.040.3223733
CGTCTGA79850.040.27186616
ACGTCTG80800.039.96536315
ACGTGGC80000.039.8881332
TCACGTG80100.039.7821830
CGGAAGA81400.039.7238354
CACGTCT82200.039.3667614
CACGTGG82500.038.70665431
GCACACG84450.038.5841911
Produced by FastQC (version 0.11.5)