FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10079305
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC286660.2844045298758198TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC35000.039.2708544
CTCGTAT35200.038.9820742
TCTCGTA35350.038.75283441
TCGTATG36250.038.10171543
ATCTCGT37400.036.68882440
CATCTCG39050.035.08029639
GATCGGA41000.034.324361
ATCGGAA42450.033.2409742
CGTCTGA42250.032.84876316
GTATGCC42300.032.63008545
ACACGTC42500.032.4962713
TCGGAAG43900.032.1428763
ACGTCTG44100.031.47074915
CGGAAGA46450.030.3287244
CACGTCT46850.029.67149214
GCACACG48050.028.88366511
CTTAGGC48700.028.453833
CACACGT49700.027.83423212
ACTTAGG53800.025.84013232
GCATCTC57100.024.42492938
Produced by FastQC (version 0.11.5)