FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7375270
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3050.015.8200451
ACACGTC3300.014.07080913
ATCGGAA4250.011.9833712
ATAGGCG3150.011.4125818
GCCACCG15200.011.14281228
CGTCTGA4450.010.77127716
CGTTGGA14650.010.7307391
ACAGGCG19150.010.71605418
TCGGAAG4600.010.4198273
GCGTGAG13850.010.38453522
CGATAAG1050.00958876110.3765891
CCCAAAG63250.010.2125321
CGTGAGC14350.010.12758823
CACCGCG6100.09.82976530
CCCAAGT26450.09.8273551
ACGGGTA1851.3047957E-59.7157869
CGGAAGA5250.09.7003644
CCCGTAA2103.544379E-69.6354041
TAGGCGT3001.0804797E-99.48702819
GAGCCAC37950.09.43834926
Produced by FastQC (version 0.11.5)