FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15874097
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAG33200.011.33950422
CGTTGGA35950.011.2967141
CGTGAGC33400.011.1817923
ACGGGAT23950.010.7085989
ACAGGCG46400.010.69962218
AAACGGG41000.010.3890667
CGGGATT21050.010.3313710
GAAACGG41050.010.3033386
CACCGCG12100.010.28812330
CCCAAAG138300.010.2233431
GCCACCG29800.010.21699328
AACGGGA33050.010.2106438
GAGCCAC85900.010.16182726
GGAAACG40750.09.8271065
CAGGCGT40000.09.56177719
AGGCGTG42100.09.54797220
CCCAAGT59100.09.4931121
TACAGGC111500.09.25490617
GGCGTGA42200.09.09883821
CCCTAAA40550.09.0878771
Produced by FastQC (version 0.11.5)