FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4763829
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGT2750.016.3532568
ACAAGCG1502.5946792E-614.99048318
CCCCGAT1604.8018046E-614.1268251
CCCGATT1604.824411E-614.12041952
AAACGGG12600.013.9197367
CGTTGGA12250.013.8385221
CGAGGTA1150.00116201413.7582991
GAAACGG13400.013.2627746
AACGGGA10650.012.4568818
ACGGGAT8350.012.1180569
GGAAACG13250.012.1053685
TATGCCG1701.344991E-411.94419945
CGTTCGA1400.005084369311.301461
CGCCAAT1400.005084369311.301461
CCGATTT1802.2592221E-411.2963353
ACGGGTA1650.001441732310.9021699
CCCGTAA2503.5002813E-610.8494011
CGCCCGG5100.010.58229435
CGAAACG1500.00852492910.5424685
CGGGATT8000.010.39964810
Produced by FastQC (version 0.11.5)