FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6408475
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA250.002108318535.99828730
CGTTGGA17700.013.98851
AAACGGG19250.012.2721437
GAAACGG21250.011.7522916
GATCGGA3702.910383E-1111.5585561
CGTATGC2758.097777E-811.45462745
TCTCGTA3602.237357E-1011.24999243
AACGGGA16400.011.1122778
CCCGAAA2656.1698483E-711.0420371
GGAAACG21200.010.9333035
TATCTCG3959.822543E-1110.82277841
ACGGGAT12800.010.7221469
CTCGTAT4001.2369128E-1010.68749244
TCGTATG4701.8189894E-1210.53198945
AACGGGT4651.8189894E-1110.1608088
CGGGATT12300.09.51166910
ACGCCCG3552.626257E-79.50659134
ATCTCGT4803.4197E-109.37499342
CCCGTTA2050.0086046038.7839091
GATTTCG8150.08.5596125
Produced by FastQC (version 0.11.5)