FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8624465
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA24050.013.9577781
AAACGGG24750.013.8156387
ACGGGAT16800.013.5242959
AACGGGA21300.013.5186388
CGGGATT14850.013.02775410
GGAAACG26550.012.8972285
GAAACGG27450.012.8685726
CACCGCG6150.010.24213530
ACCGCGC6050.010.0395931
AACGGGT5352.910383E-119.2506338
CCCGAAT7150.09.1377031
ACGGGTT4956.148184E-109.0892359
CGCCTGG13300.08.96461935
CCGCGCC7300.08.62864932
CGCGCCT3155.7269423E-58.5699533
CGTAAAC2902.1660705E-48.5455461
CGGGAAT8750.08.22696110
CGATAAT4955.252332E-88.1924231
ACCACGC17300.08.19222831
CCCCGAA3603.0075178E-58.1355311
Produced by FastQC (version 0.11.5)