FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11720979
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA27250.011.8072058
CGTTGGA33700.011.6914741
AAACGGG35000.011.5069637
ACGGGAT20500.011.30474959
GAAACGG35100.011.1536726
CGGGATT18850.010.74258410
GGAAACG35700.010.5910975
CTCGTAT5350.010.51434944
CGCCCGG9950.09.72350835
CGTATGC4904.9112714E-119.64254245
TCTCGTA5651.8189894E-129.55733243
CGAGTAT2453.848598E-49.1895671
CGCGCCC5659.094947E-129.15899333
TATCTCG6650.08.79704341
CCCGTAA5151.2769306E-98.7434711
CCCGATT4904.8694346E-98.7269962
CACCGCG7750.08.70913230
TCGTATG6301.0913936E-118.57114945
GCGTGAG26800.08.47887522
ACCGCGC7200.08.43711731
Produced by FastQC (version 0.11.5)